Regulation of connexin36 gap junction channels byn-alkanols and arachidonic acid
نویسندگان
چکیده
منابع مشابه
Functional properties of channels formed by the neuronal gap junction protein connexin36.
The expression and functional properties of connexin36 (Cx36) were examined in two communication-deficient cell lines (N2A-neuroblastoma and PC-12 cells) transfected with Cx36 and in hippocampal neurons that express the connexin endogenously. Transfected cells expressed the expected 2.9 kb Cx36 transcript and Cx36 immunoreactivity, whereas nontransfected cells were devoid of Cx36. The relations...
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Gap junction (GJ) channels composed of Connexin36 (Cx36) are widely expressed in the mammalian CNS and form electrical synapses between neurons. Here we describe a novel modulatory mechanism of Cx36 GJ channels dependent on intracellular free magnesium ([Mg(2+)]i). We examined junctional conductance (gj) and its dependence on transjunctional voltage (Vj) at different [Mg(2+)]i in cultures of He...
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This paper reports methods we have developed to solubilize gap junction channels, or connexons, from isolated gap junctions and to purify them in milligram quantities. Two sources of material are used: rat liver gap junctions and gap junctions produced by infecting insect cells with a baculovirus containing the cDNA for human liver beta 1 protein (connexin 32). Complete solubilization is obtain...
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788 with a lipidlprotein molar ratio of 100:1, a value close to that found in most biological membranes [21]. The data show that the calculation of distances between sites within the monomer of a membrane protein of a cross-sectional diameter of 5 nm based only from FET efficiency measurements is largely speculative in most cases in native biological membranes. Because the contribution of FET t...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 2013
ISSN: 0022-3751
DOI: 10.1113/jphysiol.2013.250910